Genes Essential For Growth And/or Cisplatin-resistance Of Nasopharyngeal Carcinoma Cells Were Identify Using A Pooled Genome-wide ShRNA Library Screen Approach. Briefly, C666-1 Cells Were Transduced With LentiPlex® Human Pooled ShRNA Library Containing More Than 81,000 ShRNA Constructs Targeting More Than 16,000 Human Genes (Sigma-Aldrich, St Louis, MO, USA) To Obtain A Single Viral Infection Per Cell, Left To Grow For 48 H Post-transduction Followed By Puromycin Selection (3 μg/mL For 4 Days) And Expanded Into Culture Plates For The Experiments. To Screen For Genes Associated With Proliferation, The Selected Cells Were Grown In Duplicates In 10 Cm Plates Seeded At 2 Million Cells Per Plate For An Additional 3 And 10 Days With Fresh Medium Without Puromycin. To Identify Genes Associated With Cisplatin Sensitivity, A Parallel Set Of ShRNA Library Transduced Cells Were Grown For 3 Days With Fresh Media With Cisplatin At A Dose 42.5 μM (which Was The IC50 Value For Cisplatin Of The Cells). DNA Was Then Harvested From The Cells And ShRNA Sequences Present In Each Sample Were Determined By Massively Parallel Sequencing For ShRNA And Screen Data Analysis. ShRNA With A Read Count Of Zero Was Excluded From The Analysis. Read Counts Per ShRNA In 3-day Vs. 10-day Samples (or Vs. 3-day Cisplatin Treatment For Cisplatin Sensitivity) Were Log2 Transformed And Normalized Using Non-linear Regression Via The ShRNAseq R Package. Hit Identification Was Performed Using A ShRNAseq Score Threshold Of ≥2 Or ≤ −2, And Were Targeted By At Least Two Independent ShRNAs. Please See Details In The Published Article (PMID: 33587980): Https://www.sciencedirect.com/science/article/pii/S0304383521000707 DOI: 10.1016/j.canlet.2021.02.006