Our previous findings using microarray have revealed downregulation of miR-150 and overexpression of its possible target, RAD54L in local multiple myeloma patients and cell lines. It has been shown that miRNA could bind to the target gene to regulate its expression through mRNA degradation or protein repression. RAD54L and miR-150 has been shown to play a critical role in various cancers; however, its role in the molecular pathogenesis of multiple myeloma is unknown. In this study, we aimed to study the function of RAD54L and the biological relevance of miR-150-RAD54L using in vitro model. KMS-28BM multiple myeloma cell line was used as host cell in this study. RAD54L was silenced using siRNA and the function of RAD54L in cell proliferation, apoptosis and cell cycle were measured with MTS and flow cytometry, respectively. Gene expression and protein expression were measured using RT-qPCR and ELISA methods. Statistical analysis was performed using student t-test to compared the control and treated groups, where P<0.05 was considered as statistically significance.