Unsafe transfusion practices expose patients to the risk of adverse transfusion reactions and infection transmission. Values, beliefs, attitudes, and behaviours shared among organisation members contribute to the culture of patient safety and in the workplace. A positive safety culture enhances organisation safety outcomes. Many countries focus mainly on clinical staff dealing with direct patient care when dealing with patient safety issues. This study aims to assess patient safety culture among Medical Laboratory Technologists (MLTs) in transfusion medicine services in public hospitals. This was a cross-sectional study that was conducted from October until November 2020 in all public hospitals in Malaysia. The validated bilingual version of the Hospital Survey on Patient Safety Culture (HSOPSC) was used. The inclusion criteria were all MLTs in all public hospitals involved in transfusion medicine services in their daily work and during on-calls. MLTs who were only involved with mobile support blood banks were excluded. Universal sampling was applied.